Perylene-dU-CE Phosphoramidite

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10-1591
5'-Dimethoxytrityl-5-(perylen-3-yl-ethynyl)-2'-deoxyUridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C61H57N4O8P
M.W.:
1005.10
F.W.:
564.48
Catalog Number: Select Pack Size and Format to Reveal

Description

When a benzylcarbamoyl analogue of AP-dC (G-Clamp) was synthesized, it was found that, when incorporated into an oligo, it exhibited similar fluorescence to AP-dC. However, when base-paired against the 8-oxo-dG, its fluorescence was severely quenched. Rather remarkably, however, when base paired with dG or any of the other bases, A, C or T, there was no change in fluorescence - making it a specific probe for 8-oxo-dG. By attaching pyrene or perylene to the 5 position of deoxyuridine through a triple bond, the fluorophore is electronically coupled to the deoxyuridine base. This electronic coupling of the base and the fluorophore makes the fluorescence sensitive to the base pairing of the dU portion of the molecule, allowing the discrimination between perfect and one base mismatched targets.

Details

Usage

  • Coupling: A 6 minute coupling time is recommended.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile/Dichloromethane 1:1 (v/v)
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 1-2days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1591-02 0.25 g .25grams 2.49 69.67 41.8 26.13 19 13.93 3.48
10-1591-90 100 µmol .101grams 1 20 12 7.5 5.45 4 1
10-1591-95 50 µmol .05grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1591-02 0.25 g .25grams 3.71 0.07 67.8 42.38 30.82 4.24
10-1591-90 100 µmol .101grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1591-95 50 µmol .05grams 0.75 0.07 8.6 5.38 3.91 0.54