PC Biotin Phosphoramidite

default
10-4950

1-[2-Nitro-5-(6-(N-(4,4'-dimethoxytrityl))-biotinamidocaproamidomethyl)phenyl]-ethyl-[2-cyanoethyl-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C55H72N7O9PS
M.W.:
1038.25
F.W.:
597.62
Catalog Number: Select Pack Size and Format to Reveal

Description

PC Biotin Phosphoramidite can be used to prepare 5'-biotinylated oligonucleotides suitable for capture by streptavidin in a mode similar to our popular 5' Biotin Phosphoramidite. Amino- and thiol-modified oligonucleotides have proven to be very useful for the attachment of a variety of haptens and fluorophores, as well as for the tethering of the oligonucleotides to a diversity of beads and surfaces. PC Amino-Modifier Phosphoramidite is used to prepare 5'-amino-modified oligonucleotides suitable for subsequent photocleavage. PC Spacer Phosphoramidite can be used as an intermediary to attach any modification reagent, available as a phosphoramidite, to the terminus of oligonucleotides. After photocleavage, a 5'-phosphate is generated on the DNA, rendering it suitable for further biological transformations, such as gene construction and cloning after ligation.

A versatile photocleavable DNA building block has been described by researchers in Washington University, Missouri and used in phototriggered hybridization.1 This reagent has also been used in the design of multifunctional DNA and RNA conjugates2 for the in vitro selection of new molecules catalyzing biomolecular reactions. Researchers at Bruker Daltonik in Germany have also developed genoSNIP, a method for single-nucleotide polymorphism (SNP) genotyping by MALDI-TOF mass spectrometry.3 This method uses size reduction of primer extension products by incorporation of the photocleavable linker for phototriggering strand breaks near to the 3' end of the extension primer. PC Linker can be incorporated into oligonucleotides at any position by standard automated DNA synthesis methodology. PC Linker Phosphoramidite has the added advantage in that photocleavage results in monophosphate fragments at both the 3'- and 5'-termini of the oligonucleotide fragments.

Details

Usage

  • Coupling: 2 minute coupling time recommended
  • Deprotection: PC-Biotin is slow to detritylate. If the final DMT-group is to be removed on the synthesizer, we recommend a second deblocking step.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days

Intellectual Property

Glen Research offers PC Biotin, PC Amino-Modifier and PC Spacer products in association with AmberGen, Inc. and Link Technologies, Ltd. For a commercial application license, please contact AmberGen, Inc., +617-975-0680, http://www.ambergen.com/.


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-4950-02 0.25 g .25grams 2.41 67 40.2 25.13 18.27 13.4 3.35
10-4950-90 100 µmol .104grams 1 20 12 7.5 5.45 4 1
10-4950-95 50 µmol .052grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-4950-02 0.25 g .25grams 3.59 0.07 65.4 40.88 29.73 4.09
10-4950-90 100 µmol .104grams 1.5 0.07 23.6 14.75 10.73 1.48
10-4950-95 50 µmol .052grams 0.75 0.07 8.6 5.38 3.91 0.54

References

REFERENCE(S)| 1 P. Ordoukhanian and J-S. Taylor, J. Am. Chem. Soc., 117, 9570-9571, 1995.| 2 (a) F. Hausch and A. Jäschke, Nucleic Acids Research, 2000, 28, e35.| 3 (b) F. Hausch and A. Jäschke, Tetrahedron, 2001, 57, 1261-1268.| 4 T. Wenzel, T. Elssner, K. Fahr, J. Bimmler, S. Richter, I. Thomas, and M. Kostrzewa, Nucleosides, Nucleotides & Nucleic Acids, 2003, 22, 1579-1581.