Pac-A-CE Phosphoramidite

default
10-3000
5'-Dimethoxytrityl-N-phenoxyacetyl-Adenosine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C54H68N7O9PSi
M.W.:
1018.23
F.W.:
329.21
Catalog Number: Select Pack Size and Format to Reveal

Description

Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certi cate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling ef ciency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in may be substituted with Ac-G (10-3025).

Details

Usage

  • Coupling: 12 minutes. To avoid any exchange of the iPr-Pac group on the G with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
  • Deprotection: RNA Deprotection-see bulletin
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3000-02 0.25 g .25grams 2.46 68.67 41.2 25.75 18.73 13.73 3.43
10-3000-05 0.5 g .5grams 4.91 150.33 90.2 56.38 41 30.07 7.52
10-3000-10 1.0 g 1grams 9.82 314 188.4 117.75 85.64 62.8 15.7

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3000-02 0.25 g .25grams 3.66 0.07 66 41 13.92 4
10-3000-05 0.5 g .5grams 7.33 0.07 140 88 29.21 8
10-3000-10 1.0 g 1grams 14.66 0.07 286 179 59.75 17