O6-Me-dG-CE Phosphoramidite

5'-Dimethoxytrityl-N-isobutyryl-O6-methyl-2'-deoxyGuanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C45H56N7O8P
M.W.:
853.97
F.W.:
343.24
CAS Number:
117958-69-7
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Cellular polynucleotides are alkylated by endogenous components, such as S-adenosylmethionine, or after reacting with two general classes of environmental and laboratory chemicals. SN1 chemical agents include alkylnitrosourea and N-alkyl-N-nitro-N-nitrosoguanidine that react with the N7 position of guanine, N3 of adenine, O6 of guanine, O2 or O4 of pyrimidines, and the non-phosphodiester oxygen atoms of the phosphate backbone. In contrast, SN2 chemical agents such as methyl methanesulfonate and dimethyl sulfate react primarily with the N1 position of adenine (1-Methyl-2'-deoxyadenosine) and N3 of cytosine. To avoid chain branching during synthesis when using DCI as activator, N6-Me-dA is offered with acetyl protection.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: Treat the CPG with 10% DBU in anhydrous methanol at Room Temperature for 5 days in the dark to cleave and deprotect the oligo. ||1) Prepare a solution of 10% DBU in anhydrous methanol. DBU (1,8-Diazabicyclo[5.4.0]undec-7-ene) is available from Aldrich (139009-25G) as is anhydrous methanol (322415-100ML). Note: 10% DBU in anhydrous methanol is not compatible with dmf-protecting groups.|2) Transfer the CPG to a clean, dry glass vial and add 1 mL of the 10% DBU solution.|3) Seal the vial and leave for 5 days in the dark at room temperature.|4) Transfer the contents to a micro centrifuge tube and reduce under vacuum to a small volume.|5) Add 1mL of 10mM aqueous sodium hydroxide solution and desalt or purify the oligonucleotide using standard procedures.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1070-02 0.25 g .25grams 2.93 84.33 50.6 31.63 23 16.87 4.22
10-1070-90 100 µmol .085grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1070-02 0.25 g .25grams 4.37 0.07 81 50.63 36.82 5.06
10-1070-90 100 µmol .085grams 1.5 0.07 23.6 14.75 10.73 1.48