N3-Cyanoethyl-dT

5'-Dimethoxytrityl-N3-(2-cyanoethyl)-2'-deoxyThymidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C43H52N5O8P
M.W.:
797.88
F.W.:
357.26
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

The most common side reaction during deprotection of oligonucleotides on a large scale is the alkylation of dT residues by acrylonitrile, formed by ß-elimination of the cyanoethyl phosphate protecting groups, to generate N3-cyanoethyl-dT.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1531-02 0.25 g .25grams 3.13 91 54.6 34.13 24.82 18.2 4.55
10-1531-90 100 µmol .08grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1531-02 0.25 g .25grams 4.68 0.07 87.2 54.5 39.64 5.45
10-1531-90 100 µmol .08grams 1.5 0.07 23.6 14.75 10.73 1.48