N2-Amino-Modifier C6 dG

5'-Dimethoxytrityl-N2-[6-(trifluoroacetylamino)-hex-1-yl]-2'-deoxyGuanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C48H60F3N8O8P
M.W.:
965.01
F.W.:
428.38
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Sequence Modifiers are designed for use in automated synthesis.   The carboxy-dT is hydrolyzed during deprotection and can be coupled directly to a molecule containing a primary amino group by a standard peptide coupling or via the intermediate N-hydroxysuccinimide (NHS) ester.  Amino-Modifier dA, Amino-Modifier dC, N2-Amino-Modifier dG and both Amino-Modifier dT products can be added in place of a dA, dC, dG and dT residue, respectively, during oligonucleotide synthesis.  Corresponding Amino-Modifier supports can replace their respective deoxynucleoside supports.  After deprotection, the primary amine on the C6 analogues is separated from the oligonucleotide by a spacer arm with a total of 7 -10 atoms and can be labelled or attached to an enzyme.  The C2 analogue is more suitable for the attachment of molecules designed to react with the oligonucleotide.

Details

Usage

  • Coupling: N2-Amino-Modifier C6 dG reacts in a manner identical to normal phosphoramidites. To prevent side reactions, synthesize using acetyl-protected dC. See Deprotection for further details.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. The TFA protecting group is removed during standard ammonium hydroxide deprotection. A minor side reaction during ammonia deprotection can lead to irreversibly capping 2-5% of the amine. This could be significant if multiple additions of the modifier are made. To prevent the reaction, synthesize using acetyl-protected dC and deprotect in 30% ammonia/40% methylamine 1:1 (AMA) at 65°C for 10 minutes.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1529-02 0.25 g .25grams 2.59 73 43.8 27.38 19.91 14.6 3.65
10-1529-90 100 µmol .097grams 1 20 12 7.5 5.45 4 1
10-1529-95 50 µmol .048grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1529-02 0.25 g .25grams 3.87 0.07 71 44.38 32.27 4.44
10-1529-90 100 µmol .097grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1529-95 50 µmol .048grams 0.75 0.07 8.6 5.38 3.91 0.54