N-POM Caged-dT-CE Phosphoramidite

5'-Dimethoxytrityl-N3-[[1-(6-nitro-1,3-benzodioxol-5-yl)ethoxy]methyl]-2'-deoxyThymidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C50H58N5O13P
M.W.:
967.99
F.W.:
527.38 (N-POM-dT)| FW: 304.20 (dT)
CAS Number:
942218-71-5
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Glen Research's interest lies in the preparation of caged oligonucleotides whose function is restored after uncaging by UV light at a wavelength that causes no DNA damage. The Deiters group at North Carolina State University has described NPOM-Caged-dT, where the nucleobase is caged with the photolabile group, 6-nitropiperonyloxymethyl (NPOM), which can be removed using UV light at 365nm. Oligonucleotides containing NPOM-Caged-dT every five or six bases do not hybridize to their complementary strand. Photo-uncaging of the caged oligonucleotide is then easily carried out with UV light at 365 nm for seconds to minutes to restore the activity of the oligonucleotide.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry. Light sensitive material.
Stability 1-2 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1534-02 0.25 g .25grams 2.58 72.67 43.6 27.25 19.82 14.53 3.63
10-1534-90 100 µmol .097grams 1 20 12 7.5 5.45 4 1
10-1534-95 50 µmol .048grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1534-02 0.25 g .25grams 3.85 0.07 70.6 44.13 32.09 4.41
10-1534-90 100 µmol .097grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1534-95 50 µmol .048grams 0.75 0.07 8.6 5.38 3.91 0.54