Methacrylate C6 Phosphoramidite

Methacrylate C6 Phosphoramidite is a terminus modifier that attaches a methacrylate functional group to an oligonucleotide.

Product Specifications

Formula:
C19H36N3O3P
M.W.:
385.48
F.W.:
248.24
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Methacrylate C6 Phosphoramidite is a terminus modifier that attaches a methacrylate functional group to an oligonucleotide.  Methacrylate-modified oligonucleotides can be reacted with thiol-modified surfaces or incorporated into polyacrylamide gels. Methacrylate-modified oligonucleotides have been used to generate oligonucleotide labeled hydrogels, microspheres, microarrays, and functionalized polymeric surfaces, among other applications. These oligonucleotide-labeled surfaces and polymers have been used for purification, hybridization detection, PCR amplification, affinity capture, aptamers, sequencing, and biosensors.  Recent applications of methacrylate-labeled oligonucleotides include aptamer-based hydrogels, molecular imprinting, and Expansion Microscopy.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Acetonitrile, anhydrous
Storage Freezer storage, -10 to -30C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1891-90 100 µmol 0.04 0.1 20 12 7.5 5.45 4 1
10-1891-02 0.25 g 0.25 0.1 6.49 203 121.8 76.13 55.36 40.6

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1891-90E 100 µmol 0.04 1.5 0.07 23.6 14.75 10 1.48
10-1891-02E 0.25 g 0.25 1.5 0.07 187.2 117 85.09 11.7

References

1. F.N. Rehman, et al., Nucl Acid Res, 1999, 27, 649-655.

2. J. Liu, et al., Anal Bioanal Chem, 2012, 402, 187-94.

3. Z. Zhang and J. Liu, Small, (In Press), 201805246.

4. N. Tsanov, et al., Nucleic Acids Res, 2016, 44, e165.

5. R. Liu, et al., ACS Applied Materials & Interfaces, 2015, 7, 6982-6990.

6. F. Chen, P.W. Tillberg, and E.S. Boyden, Science (New York, N.Y.), 2015, 347, 543-548.

7. E.S. Boyden, 2016, http://bit.ly/2LgJz3d