I-CE Phosphoramidite

5'-Dimethoxytrityl-Inosine, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C46H61N6O8PSi
M.W.:
885.08
F.W.:
330.19
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Inosine and 5-Methyl-Uridine are useful for analyzing RNA structure and activity relationships.

Details

Usage

  • Coupling: 12 minute coupling time recommended.
  • Deprotection: RNA deprotection- seeTechnical Bulletin
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3040-02 0.25 g .25grams 2.82 80.67 48.4 30.25 22 16.13 4.03
10-3040-90 100 µmol .089grams 1 20 12 7.5 5.45 4 1
10-3040-95 50 µmol .044grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3040-02 0.25 g .25grams 4.22 0.07 78 48.7 16.25 4.8
10-3040-90 100 µmol .089grams 1.5 0.07 23.6 14.7 4.92 1.4
10-3040-95 50 µmol .044grams 0.75 0.07 8.6 5.3 1.79 0.5