Glen Research is dedicated to the production of analogues of the standard deoxynucleosides as phosphoramidites for oligonucleotide synthesis. To our existing line, we are happy to add the following five monomers which have been most requested by researchers. It would be short sighted for us to predict all of the promising applications for these monomers, but we will attempt to give some indication of their potential uses.
The 7-deaza analogues of 2'-deoxyAdenosine (1) and 2'-deoxyGuanosine (2) have been requested for physical studies related to the interaction of enzymes and DNA. Further uses may be found in the examination of DNA structure, especially with the current interest in the formation and structure of triplex DNA.
Cordycepin (3'-deoxyAdenosine) (3) offers some potential interest for the Deoxynucleoside Analogues formation of non standard 2'-5' linkages which may exhibit resistance to nucleases and restriction enzymes. The product will also be available attached to CPG to produce oligonucleotides with cordycepin at the 3' terminus. Oligos with cordycepin at the 3' terminus may be useful in blocking chain extension by polymerases.
N6-Methyl-2'-deoxyAdenosine (4) may find its utility in carcinogenesis studies for which we currently offer the 06-methyl-2'-deoxyGuanosine analogue. 5-lodo-2' -deoxyCytidine (5) joins our already extensive list of halogenated deoxynucleosides. The iodo analogues seem to be most interesting for structural studies using X-ray crystallography.
We are now maintaining a comprehensive list of analogues separate from our catalog since the availability of existing products may vary while new products are always in planning. Request our Minor Bases Catalog Insert for an up-to-date listing of 2'-deoxynucleoside analogues. (Insert is no longer available.)
Please see our current line of Nucleoside Analog Phosphoramidites