GAC Trimer Phosphoramidite

default
13-1201
5'-Dimethoxytrityl-N2-isobutyryl-2'-deoxyGuanosine-3'->5'-o-chlorophenyl-phosphoryl-N6-benzoyl-2'-deoxyAdenosine-3'->5'-o-chlorophenyl-phosphoryl-N4-benzoyl-2'-deoxyCytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C89H92Cl2N15O21P3
M.W.:
1869.5
Catalog Number: Select Pack Size and Format to Reveal

Description

All of the trimers are available individually so the researchers can prepare custom trimer mixes. Two pre-made catalog trimer mixes are available: 13-1991-xx, for incorporating all 20 amino acid codons equally into a sequence and 13-1992-xx, for incorporating 19 amino acid codons (-Cys). For a custom trimer mix of a particular subset of codons or a trimer mix that represents a set of trimers that is biased toward a particular codon or codons, please contact [email protected] for a quotation and projected delivery date.

Details

Usage

  • Coupling: 15 minute coupling time recommended.
  • Deprotection: 30% NH4OH for 17 hours at room temperature followed by an additional 4 hours at 55°C.
Specifications
Diluent Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v)
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
13-1201-90 100 µmol .187grams 1 20 12 7.5 5.45 4 1
13-1201-95 50 µmol .093grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
13-1201-90 100 µmol .187grams 1.5 0.07 23.6 14.75 10.73 1.48
13-1201-95 50 µmol .093grams 0.75 0.07 8.6 5.38 3.91 0.54