1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-3,3-dimethyl-2-[5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)penta-2,4-dienylidene]indole-5-sulfonic acid, potassium salt
Cyanine 5 is a popular indocyanine fluorophore that exhibits low, non-specific binding and relatively pH-independent fluorescence. This NHS ester version allows for post-deprotection labeling, and unlike the phosphoramidite version, contains 2 sulfonate substitutions directly on the indocyanine nuclei. The sulfonates make the Cyanine 5 less susceptible to aggregation and do not significantly change the fluorescent properties of the dye.
Details
Usage
Coupling: For labeling 0.2 µmoles of an amino-modified oligonucleotide, dissolve 3.9 mg of the sulfoCyanine 5 NHS Ester in 100 µL DMSO (or DMF). Dissolve the amino-modified oligonucleotide in 500 µL 0.1 M carbonate/bicarbonate buffer (pH 9). Add 20 µL of the NHS Ester solution and vortex. Let react for 60 min at room temperature and desalt.
