Quasar®570 and Quasar®670 dyes are two cyanine derivatives of Cyanine 3 and Cyanine 5, respectively. These two fluorophores differ in structure simply by the number of carbons in the conjugated polyene linkage. Cyanine dyes are a popular oligonucleotide modification and are commonly used for imaging, flow cytometry, and genomic applications. With Quasar®570-dT and Quasar®670-dT, these dyes can be incorporated internally without disrupting base pairing or stacking. Monomers can be inserted as a replacement for a dT residue.
Particularly for Quasar® 670-dT, the use of UltraMILD monomers is preferred. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMILD Cap Mix A (40-4210-xx/40-4212-xx). 0.02M Iodine for oxidation is recommended.
Specifications | |
---|---|
Diluent | Anhydrous Acetonitrile |
Storage | Freezer storage, -10 to -30°C, dry |
Stability | 1-2 Days |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
---|---|---|---|---|---|---|---|---|---|
LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
10-5953-95 | 50 µmol | 0.08 | 0.5 | 3.33 | 2 | 1.25 | 0.91 | 0.67 | 0.17 |
10-5953-90 | 100 µmol | 0.15 | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
10-5953-02 | 0.5 g | 0.25 | 1.67 | 42.33 | 25.4 | 15.88 | 11.55 | 8.47 | 2.12 |
Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
---|---|---|---|---|---|---|---|---|
Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
10-5953-95E | 50 µmol | 0.08 | 0.75 | 0.07 | 8.6 | 5.38 | 3.91 | 0.54 |
10-5953-90E | 100 µmol | 0.15 | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
10-5953-02E | 0.25 g | 0.25 | 2.49 | 0.07 | 43.4 | 27.13 | 19.73 | 2.71 |