5'-O-(4,4'-Dimethoxytrityl)-1'-(1,3-diaza-2-oxophenoxazin-1-yl)-2’-O-TBDMS-β-D-ribofuranosyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
The bright fluorescent tricyclic cytosine analogues tC and tCO stand out among fluorescent bases due to their virtually unquenched fluorescence inside single- or double-stranded DNA. Until recently, this family of tricyclic cytosines had only been studied and used in DNA contexts and, importantly, introduced as possible donors of the first DNA base analogue FRET-pair with tCnitro. Fluorescent base analogues for RNA are limited in number compared to their DNA counterparts. To facilitate the application of such analogues, characterization of their structural and dynamics behavior in RNA compared to the corresponding natural nucleoside is important. We now introduce the tCO ribonucleoside, which has been incorprated into a range of RNA sequences, where it was shown to be a very potent and useful fluorophore in this context.1 Glen Research offers this useful fluorescent ribonucleoside analogue in cooperation with ModyBase HB.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Storage | Freezer storage, -10 to -30°C, dry |
| Stability | 1-2 Days |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-3517-02 | 0.25 g | 0.25 | 2.63 | 74.33 | 44.6 | 27.88 | 20.27 | 14.87 | 3.72 |
| 10-3517-90 | 100 µmol | 0.1 | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-3517-95 | 50 µmol | 0.05 | 0.5 | 3.33 | 2 | 1.25 | 0.91 | 0.67 | 0.17 |
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-3517-02 | 0.25 g | 0.25 | 3.93 | 0.07 | 72.2 | 45.13 | 32.82 | 4.51 |
| 10-3517-90 | 100 µmol | 0.1 | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
| 10-3517-95 | 50 µmol | 0.05 | 0.75 | 0.07 | 8.6 | 5.38 | 3.91 | 0.54 |
(1) Füchtbauer, A.F., Preus, S., Börjesson, K., McPhee, S.A., Lilley D.M.J., Wilhelmsson, L.M., Sci. Rep., 2017, 7, 2393.
