Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups.
Pyrrolo-C is a fluorescent nucleoside whose fluorescence is sensitive to its environment and is ideal for probing RNA structure. It base-pairs as a normal C nucleotide. It is highly fluorescent and its excitation and emission are well to the red of most fluorescent nucleotide analogs, which eliminates or reduces background fluorescence from proteins. Pyrrolo-CTP has potential uses in biological assay development.
Details
Usage
Coupling: 6 minute coupling time recommended
Deprotection: Ethanolic Ammonium Hydroxide (1:3) at room temperature for 24 hours or as required by the nucleobases. Remove silyl protecting group according to standard RNA procedures.
Specifications
Diluent
Anhydrous Acetonitrile
Storage
Freezer storage, -10 to -30°C, dry
Stability
2-3 days
Intellectual Property
This product is covered by US Patent No.: 7,144,995.
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
