Ferrocene-dT-CE Phosphoramidite

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10-1576
5'-dimethoxytrityloxy-5-[N-acetyl ferrocenyl-aminohexyl)-3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C60H73FeN6O10P
M.W.:
1125.07
F.W.:
684.45
Catalog Number: Select Pack Size and Format to Reveal

Description

With an excellent stability profile, ferrocene has always attracted considerable interest for DNA labelling to generate probes for electrochemical detection. Based on our Amino-Modifier C6-dT structure, Ferrocene-dT is easily added to oligonucleotides with no disruption of regular hybridization behavior. Multiple incorporations into an oligonucleotide probe are also simply achieved. Oligonucleotides are deprotected using standard techniques. Ferrocene oligonucleotides should be stored under Argon and aqueous solutions should be degassed immediately.

Details

Usage

  • Coupling: 3 minute coupling time recommended
  • Deprotection: Synthesize using acetyl-protected dC (10-1015-xx) and deprotect in 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA) at 65°C for 10 minutes OR Synthesize using UltraMild monomers and Cap A (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx, Cap Mix A (40-4210-xx/ 40-4212-xx) and deprotect in Ammonium Hydroxide for 2 hours at room temperature.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1576-02 0.25 g .25grams 2.22 60.67 36.4 22.75 16.55 12.13 3.03
10-1576-90 100 µmol .113grams 1 20 12 7.5 5.45 4 1
10-1576-95 50 µmol .056grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1576-02 0.25 g .25grams 3.32 0.07 60 37.5 27.27 3.75
10-1576-90 100 µmol .113grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1576-95 50 µmol .056grams 0.75 0.07 8.6 5.38 3.91 0.54