Palmitate Phosphoramidite

Palmityl-6-aminohexyl [(2-cyanoethyl)-(N,N-diisopropyl)]phosphoramidite

Product Specifications

Formula:
C31H62N3O3P
M.W.:
555.83
F.W.:
417.57
CAS Number:
1772522-18-5
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Palmitate Phosphoramidite is a saturated fatty acid with a 16-carbon backbone. This 5’-modification is a lipophilic group. When incorporated into a synthetic oligonucleotide, Palmitate conjugates offer enhanced cellular uptake and delivery to extrahepatic tissues.

Details

Usage

  • Coupling: 6 minute coupling time recommended
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v)
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1978-95 50 µmol .028grams 0.5 3.33 2 1.25 0.91 0.67 0.17
10-1978-90 100 µmol .056grams 1 20 12 7.5 5.45 4 1
10-1978-02 0.25 g 0.25grams 4.5 136.67 82 51.25 37.27 27.33 6.83

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1978-95E 50 µmol .028grams 0.75 0.07 8.6 5.38 3.91 0.54
10-1978-90E 100 µmol .056grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1978-02E 0.25 g 0.25grams 6.71 0.07 127.8 79.88 58.09 7.99