EDTA-C2-dT-CE Phosphoramidite

5'-Dimethoxytrityl-5-[N-ethylenediaminetriethylacetate,monoacetylaminoethyl-3-acrylimido]-2'-deoxyUridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C60H81N8O16P
M.W.:
1201.32
F.W.:
676.53
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

EDTA-C2-dT phosphoramidite contains the triethyl ester of EDTA which allows sequence-specific cleavage of single- and double-stranded DNA and RNA. The cleavage reaction is only initiated once Fe(II) and dithiothreitol are added and so is readily controlled. Coupling of EDTA-dT is normal but cleavage and deprotection should be carried out with sodium hydroxide in aqueous methanol (0.4M NaOH in methanol/water 4:1) overnight at room temperature.

Details

Usage

  • Coupling: 6 minute coupling time recommended.
  • Deprotection: 0.4M methanolic sodium hydroxide (methanol:water 4:1) at room temperature for 17 hours.|Note: NaOH is not compatible with dmf protecting groups.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1059-02 0.25 g .25grams 2.08 56 33.6 21 15.27 11.2 2.8
10-1059-90 100 µmol .12grams 1 20 12 7.5 5.45 4 1
10-1059-95 50 µmol .06grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1059-02 0.25 g .25grams 3.11 0.07 55.8 34.88 25.36 3.49
10-1059-90 100 µmol .12grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1059-95 50 µmol .06grams 0.75 0.07 8.6 5.38 3.91 0.54