dX-CE Phosphoramidite

5'-(4,4'-Dimethoxytrityl)-2,6-O-bis(2-(4-nitrophenyl)ethyl)-2'-deoxyXanthosine,3’-[(2-cyanoethyl)-N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C56H61N8O12P
M.W.:
1069.10
F.W.:
330.19
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

2'-DeoxyXanthosine (dX) is a naturally occurring nucleoside that may be derived from oxidative deamination of 2'-deoxyGuanosine (dG). dX has a similar bonding pattern to thymidine and it may base pair with dA, with such purine-purine interactions causing duplex distortion. dX also featured in attempts to extend the genetic alphabet with a new base pair of dX and pyrimidine-2,4-diamine nucleoside. dX has also interested researchers in the field of DNA damage and repair since it is a product of nitric oxide-induced mutagenesis.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: With the oligonucleotide still attached to the support, treat with 1 M DBU in anhydrous ACN for 2-3 minutes, followed by 17 hours using fresh 1 M DBU at room temperature. Rinse support with ACN and then deprotect as required by nucleobases. Note, the dX nucleoside is susceptible to depurination though short exposure to acid is tolerated - e.g. Glen-Pak purification.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1537-02 0.25 g .25grams 2.34 64.67 38.8 24.25 17.64 12.93 3.23
10-1537-90 100 µmol .107grams 1 20 12 7.5 5.45 4 1
10-1537-95 50 µmol .053grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1537-02 0.25 g .25grams 3.49 0.07 63.4 39.63 28.82 3.96
10-1537-90 100 µmol .107grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1537-95 50 µmol .053grams 0.75 0.07 8.6 5.38 3.91 0.54