dW-CE Phosphoramidite

3-Methyl-2-pivaloyloxy-5-[2'-deoxy-3'-O-(2-cyanoethyl-N,N-diisopropylamino)-phosphino-5'-O-(dimethoxytrityl)-beta-D-ribofuranos-1'-yl]-6-(triisopropylsilylethynyl)-pyridine

Product Specifications

Formula:
C57H78N3O8PSi
M.W.:
992.30
F.W.:
311.23
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

dW is a C-nucleoside that acts as a strong adenine base paring analog. In addition to the typical two hydrogen bonds found between T and A, dW can also interact with A via van der Waals forces. The result is a dW–A interaction that approaches the strength of a C–G base pair while also exhibiting enhanced base-pairing fidelity. dW can be used in place of T as a single substitution or a complete replacement for oligonucleotide hybridization applications. Please see Glen Report article 31.21 and Glen Report article 31.22 for more details.

Details

Usage

  • Coupling: A 15 minute coupling time is recommended. Deblocking should be carried out with 3% dichloroacetic acid in dichloromethane.
  • Deprotection: Deprotection must be carried out in the absence of heat. Otherwise, all standard conditions should be compatible. Typical conditions would be ammonium hydroxide or ammonium hydroxide/aqueous methylamine 1:1 at room temperature.
    TIPS removal: After deprotection, dry down oligo and take up in 0.5 mL DMF. Add 0.1 mL TBAF. Vortex and keep at room temperature for 30 minutes. Mix with 0.5 mL 2 M TEAA and desalt on Glen Gel-Pak™ column or equivalent.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 Days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1527-02 0.25 g .25grams 2.52 70.67 42.4 26.5 19.27 14.13 3.53
10-1527-90 100 µmol .099grams 1 20 12 7.5 5.45 4 1
10-1527-95 50 µmol .05grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1527-02 0.25 g .25grams 3.76 0.07 68.8 43 31.27 4.3
10-1527-90 100 µmol .099grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1527-95 50 µmol .05grams 0.75 0.07 8.6 5.38 3.91 0.54