5'-Trimethoxystilbene Cap Phosphoramidite


Product Specifications

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New cap structures allow for the preparation of hybridization probes with increased affinity for complementary sequences. The monomers used to prepare capped oligonucleotides are phosphoramidites that can be readily introduced via automated DNA synthesis at the end of solid phase syntheses. The caps favor the formation of stable Watson-Crick duplexes by stacking on the terminal base pair.

Melting point increases of over 10°C per modification can be realized for short duplexes.1,2 The caps fit canonical Watson-Crick base pairs and do not stack well on mismatched base pairs. This leads to increased base pairing selectivity at the terminal and the penultimate position of oligonucleotides featuring the caps. Base pairing fidelity is usually low at the termini, where fraying occurs frequently in the absence of caps. The beneficial effects of the caps are also realized when longer target strands are bound, so there is no need for blunt ends for the duplexes formed.1,2 The caps, when attached to the 5' terminus of an oligonucleotide, also facilitate purification as their lipophilicity leads to prolonged retention on reversed phase columns or cartridges. Finally, capping of termini may discourage the degradation of oligonucleotides by exonucleases. 3'-Uaq Cap CPG, a Uridine support modified with a 2'-anthraquinone residue, is the most effective oligonucleotide cap known to date.3,4 For short hybrid duplexes between DNA probes and RNA target strands, the increase in Tm is up to 18 °C and the modification is effective in increasing the Tm of DNA:DNA, RNA:RNA, and DNA:RNA hybrid duplexes. 3'-Uaq Cap also increases probe specificity by depressing the melting point of terminal mismatches.



  • Coupling: No changes needed from standard method recommended by synthesizer.
  • Deprotection: No changes needed from standard methold recommended by synthesizer.
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 6 months

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1986-02 0.25 g .25grams 4.37 132.33 79.4 49.63 36.09 26.47 6.62
10-1986-90 100 µmol .057grams 1 20 12 7.5 5.45 4 1


Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1986-02 0.25 g .25grams 6.53 0.07 124.2 77.63 56.45 7.76
10-1986-90 100 µmol .057grams 1.5 0.07 23.6 14.75 10.73 1.48


REFERENCES| 1 Dogan, Z.; Paulini, R.; Rojas Stütz, J. A.; Narayanan, S.; Richert, C. J. Amer. Chem. Soc. 2004, 126, 4762- 4763.| 2 Narayanan, S.; Gall, J.; Richert, C. Nucleic Acids Res. 2004, 32, 2901- 2911.| 3 A. Patra, C. Richert, J. Amer. Chem. Soc., 2009, 131, 12671-12681.| 4 C. Ahlborn, K. Siegmund, C. Richert, J. Amer. Chem. Soc., 2007, 129, 15218-15232.