3-Methyl-2-pivaloyloxy-5-[2'-deoxy-3'-O-(2-cyanoethyl-N,N-diisopropylamino)-phosphino-5'-O-(dimethoxytrityl)-beta-D-ribofuranos-1'-yl]-6-(triisopropylsilylethynyl)-pyridine
dW is a C-nucleoside that acts as a strong adenine base pairing analog. In addition to the typical two hydrogen bonds found between T and A, dW can also interact with A via van der Waals forces. The result is a dW–A interaction that approaches the strength of a C–G base pair while also exhibiting enhanced base-pairing fidelity. dW can be used in place of T as a single substitution or a complete replacement for oligonucleotide hybridization applications. Please see Glen Report article 31.21 and Glen Report article 31.22 for more details.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Storage | Freezer storage, -10 to -30°C, dry |
| Stability | 1-2 Days |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-1527-02 | 0.25 g | .25grams | 2.52 | 70.67 | 42.4 | 26.5 | 19.27 | 14.13 | 3.53 |
| 10-1527-90 | 100 µmol | .099grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1527-95 | 50 µmol | .05grams | 0.5 | 3.33 | 2 | 1.25 | 0.91 | 0.67 | 0.17 |
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-1527-02 | 0.25 g | .25grams | 3.76 | 0.07 | 68.8 | 43 | 31.27 | 4.3 |
| 10-1527-90 | 100 µmol | .099grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
| 10-1527-95 | 50 µmol | .05grams | 0.75 | 0.07 | 8.6 | 5.38 | 3.91 | 0.54 |
