Cleavable linkers have a wide range of application uses. We now offer Universal-CE, a dual-purpose linker capable of revealing 3’-OH termini or serving as a 5’-amino modifier C3 upon cleavage. Used as a universal phosphoramidite, the resulting oligonucleotides with a 3’-OH may be crucial for downstream applications, such as primer extension or ligation assays. Used as an amino modifier, the resulting oligonucleotides would be identical to those generated with 5’-Amino-Modifier C3-TFA.
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Deprotect using Ammonium Hydroxide:MethylAmine (AMA) 1:1 for 1 hour at 65 °C or Ammonium Hydroxide for 8 hours at 55 °C. For sensitive minor bases or dyes, use 50 mM Potassium Carbonate in Methanol in 17 hours at room temperature or with Tert-Butylamine/water 1:3 (v/v) for 4 hours at 60 °C.
Freezer storage, -10 to -30°C, dry
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.