dT-H-Phosphonate, TEA Salt

5'-Dimethoxytrityl-2'-deoxyThymidine,3'-H-phosphonate, TEA salt

Product Specifications

Formula:

C₃₇H₄₈N₃O₉P

M.W.:

709.78

F.W.:

304.20

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Description

Details

Technical Documents

Dilution/Coupling Data

Product FAQs

Description

Glen Research H-Phosphonates are analyzed by HPLC and are synthesis-tested. H-Phosphonates are especially useful for the preparation of modified internucleotide linkages which are unattainable by phosphoramidite chemistry. The most popular application is the preparation of radiolabelled phosphorothioates, since the sulfurization reaction is carried out off the synthesizer.

Details

Usage

Coupling: H-Phosphonate Synthesis Protocol
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Specifications
Diluent	Acetonitrile/Pyridine (1:1)
Storage	Refrigerated storage, maximum of 2-8°C, dry
Stability	~1week

Technical Documents

Safety Data Sheet

H-Phosphonates

Glen Report 4-11: Introduction

Glen Report 4-12: RNA Synthesis - Options for 2'-0H Protection

Glen Report 4-14: Literature Review

Glen Report 1-13: A Practical Application of Nucleoside H-Phosphonates for Point Modification of Oligonucleotides

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1230-02	0.25 g	.25grams	21	686.67	412	257.5	187.27	137.33	34.33
10-1230-05	0.5 g	.5grams	42	1386.67	832	520	378.18	277.33	69.33

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1230-02	0.25 g	.25grams	5	0.07	93.6	58.5	42.55	5.85
10-1230-05	0.5 g	.5grams	10	0.07	193.6	121	88	12.1

Product FAQs

What are the concentrations of the solutions used on an ABI for H-phosphonate chemistry?
1. Adamantantanecarbonyl chloride - 2% w/v in 5%pyridine/acetonitrile.
2. Isopropyl phosphite - 1% in acetonitrile/pyridine (50:50)
3. Oxidation: Following synthesis
  1. React support with 4% Iodine in THF/Pyridine/Water ( 8:1:1) ~2mL for 4minutes
  2. Remove solution from support
  3. React support with mixture of 4% Iodine in THF/Pyridine/Water ( 8:1:1) (~1mL) and TEA/H2O/THF (8:1:1) (~1mL) for 3 minutes
  4. Flush column with acetonitrile and blow dry support

dT-H-Phosphonate, TEA Salt

Description