dT-CE Phosphoramidite

5'-Dimethoxytrityl-2'-deoxyThymidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C40H49N4O8P
M.W.:
744.83
F.W.:
304.20
CAS Number:
98796-51-1
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Glen Research CE (B-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination and tested to ensure a tight fit on synthesizers.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Controlled room temperature or lower, dry
Stability Normal


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1030-02 0.25 g .25grams 3.36 98.67 59.2 37 26.91 19.73 4.93
10-1030-05 0.5 g .5grams 6.71 210.33 126.2 78.88 57.36 42.07 10.52
10-1030-10 1.0 g 1grams 13.43 434.33 260.6 162.88 118.45 86.87 21.72
10-1030-20 2.0 g 2grams 26.85 881.67 529 330.63 240.45 176.33 44.08
10-1030-40 4.0 g 4grams 53.7 1776.67 1066 666.25 484.55 355.33 88.83

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1030-C2 0.25 g 0.25grams 5.01 0.07 97 60.63 44.09 6.06
10-1030-C5 0.5 g 0.5grams 10.02 0.07 197.2 123.25 89.64 12.33
10-1030-1C 1.0 g 1grams 20.04 0.07 397.6 248.5 180.73 24.85
10-1030-2C 2.0 g 2grams 40.08 0.07 798.4 499 362.91 49.9