DNP-TEG Phosphoramidite

1-Dimethoxytrityloxy-3-O-[N-(2,4-dinitrophenyl)-3-N-aminopropyl-(triethyleneglycol)]-glyceryl-2-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

Product Specifications

Formula:
C48H64N5O13P
M.W.:
950.00
F.W.:
509.41
CAS Number:
1027512-01-1
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

An analytical test based on detection of 2,4-dinitrophenyl (DNP) labelled oligonucleotides with anti-DNP antibodies has been proposed. We have chosen the branched triethylene glycol (TEG) spacer in our version of DNP phosphoramidite since it can be added once or several times to the 3' or 5' terminus.

Details

Usage

  • Coupling: 12-15 minute coupling time. To maintain label yield, carry out the synthesis DMT-on.
  • Deprotection: To maintain label yield as a 5' modifier, cleave and deprotect as required by nucleobases and then remove the DMT group. Alternatively, treat column with 10% diethylamine in acetonitrile for 2 minutes at room temperature (2x) to remove cyanoethyl protecting groups and rinse with acetonitrile. At this point, the DMT group may be safely removed without loss of label during deprotection.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1985-02 0.25 g .25grams 2.63 74.33 44.6 27.88 20.27 14.87 3.72
10-1985-90 100 µmol .095grams 1 20 12 7.5 5.45 4 1
10-1985-95 50 µmol .048grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1985-02 0.25 g .25grams 3.93 0.07 72.2 45.13 32.82 4.51
10-1985-90 100 µmol .095grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1985-95 50 µmol .048grams 0.75 0.07 8.6 5.38 3.91 0.54


Product FAQs

  • Can oligonucleotides modified at the 5'-terminus with, for example, biotin be phosphorylated with kinase?