dmf-dG-CE Phosphoramidite

5'-Dimethoxytrityl-N-dimethylformamidine-2'-deoxyGuanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:

C₄₃H₅₃N₈O₇P

M.W.:

824.92

F.W.:

329.21

CAS Number:

330628-04-1

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Technical Documents

Description

Details

Dilution/Coupling Data

Technical Documents

Safety Data Sheet

Glen Report 19.24: New Lines of Monomers - HT for High Throughput and LC for Low Cost RNA

Glen Report 8.13: New Protecting Groups for DNA, RNA and 2'-OMe-RNA Monomers

Description

Glen Research CE (B-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination, and each vial type is thoroughly tested or inspected to ensure a tight fit on synthesizers.

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Specifications
Diluent	Anhydrous Acetonitrile
Storage	Controlled room temperature or lower, dry
Stability	Normal

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1029-02	0.25 g	.25grams	3.03	87.67	52.6	32.88	23.91	17.53	4.38
10-1029-05	0.5 g	.5grams	6.06	188.67	113.2	70.75	51.45	37.73	9.43
10-1029-10	1.0 g	1grams	12.12	390.67	234.4	146.5	106.55	78.13	19.53
10-1029-20	2.0 g	2grams	24.24	794.67	476.8	298	216.73	158.93	39.73
10-1029-40	4.0 g	4grams	48.49	1603	961.8	601.13	437.18	320.6	80.15

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1029-C2	0.25 g	.25grams	4.52	0.07	87.2	54.5	39.64	5.45
10-1029-C5	0.5 g	.5grams	9.05	0.07	177.8	111.13	80.82	11.11
10-1029-1C	1.0 g	1grams	18.09	0.07	358.6	224.13	163	22.41
10-1029-2C	2.0 g	2grams	36.19	0.07	720.6	450.38	327.55	45.04