dC-CPG 2000

5'-Dimethoxytrityl-N-benzoyl-2'-deoxyCytidine, 3'-succinoyl-long chain alkylamino-CPG 2000

Product Specifications

F.W.:
289.18
Pore Size:
2000Å
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

All Glen Research CPG supports use the standard long chain alkylamino (lcaa) linker but differ in the glass pore size, 500Å, 1000Å or 2000Å. The 500Å support is appropriate for shorter sequences, while the 1000Å supports perform better in the synthesis of longer (>30-mer) DNA sequences. The 2000Å support is best for very long (>150-mer) oligonucleotides. We have instituted an additional QC test for supports to show the length of oligo that can be prepared before a drop-off in coupling due to steric effects begins to occur. The drop-off point is recorded in the Certificate of Analysis. All Glen Research supports are fully end-capped to ensure that the CPG surface is totally inert, thereby avoiding the introduction of impurity sequences containing deletions at the 3'-terminus.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: Deprotect using the protocol required by the nucleobases.
Specifications
Storage Refrigerated storage, maximum of 2-8°C, dry

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
20-2012-01 0.1 g .1grams 0 0 0 0 0 0 0
20-2012-02 0.25 g .25grams 0 0 0 0 0 0 0
20-2012-10 1.0 g 1grams 0 0 0 0 0 0 0
20-2112-42 4 x 0.2 µmol .034grams 0 0 0 0 0 0 0
20-2212-42 4 x 0.2 µmol .034grams 0 0 0 0 0 0 0

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
20-2012-01 0.1 g .1grams 0 0 0 0 0 0
20-2012-02 0.25 g .25grams 0 0 0 0 0 0
20-2012-10 1.0 g 1grams 0 0 0 0 0 0
20-2112-42 4 x 0.2 µmol .034grams 0 0 0 0 0 0
20-2212-42 4 x 0.2 µmol .034grams 0 0 0 0 0 0

References


Frequently Asked Technical Question

QUESTION: How do the 1000Å and 2000Å supports compare in the synthesis of long oligos? Do you recommend any changes to the cycle for long oligos?

RESPONSE:In one comparison several years ago, a customer compared 1000Å, 2000Å and a low-loaded 500Å CPG for the synthesis of 200 and 400mers. Only in the case of the 2000Å CPG could the 200mer product be seen on a gel although the other supports did make product since it could be amplified by PCR. The 400mers could not be seen but could be amplified by PCR.

For the synthesis of long oligos, we recommend increasing the coupling wait step from 15 seconds to at least 30 seconds. I believe this is important later in the synthesis. Also, we recommend the use of DMAP in the Cap B solution or, if methylimidazole has to be used, increasing the capping wait step to 45 seconds. With incomplete capping, you are going to be making oligos contaminated with deletion mutations. I know DMAP has been accused of causing base modification of dG sites leading to fluorescent bands on gels but it is still the most effective capping activator. I also would recommend, if possible



Product FAQs

  • How do the 1000Å and 2000Å supports compare in the synthesis of long oligos? Do you recommend any changes to the cycle for long oligos?