dC-CPG 500

5'-Dimethoxytrityl-N-benzoyl-2'-deoxyCytidine, 3'-succinoyl-long chain alkylamino-CPG 500

Product Specifications

F.W.:
289.18
Pore Size:
500Å
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

All Glen Research CPG supports use the standard long chain alkylamino (lcaa) linker but differ in the glass pore size, 500Å, 1000Å or 2000Å. The 500Å support is appropriate for shorter sequences, while the 1000Å supports perform better in the synthesis of longer (>30-mer) DNA sequences. The 2000Å support is best for very long (>150-mer) oligonucleotides. We have instituted an additional QC test for supports to show the length of oligo that can be prepared before a drop-off in coupling due to steric effects begins to occur. The drop-off point is recorded in the Certificate of Analysis. All Glen Research supports are fully end-capped to ensure that the CPG surface is totally inert, thereby avoiding the introduction of impurity sequences containing deletions at the 3'-terminus.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: Deprotect using the protocol required by the nucleobases.
Specifications
Storage Refrigerated storage, maximum of 2-8°C, dry

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
20-2010-01 0.1 g .1grams 0 0 0 0 0 0 0
20-2010-02 0.25 g .25grams 0 0 0 0 0 0 0
20-2010-10 1.0 g 1grams 0 0 0 0 0 0 0
20-2110-13 1 x 10 µmol .23grams 0 0 0 0 0 0 0
20-2210-14 1 x 15 µmol .345grams 0 0 0 0 0 0 0
20-2110-42 4 x 0.2 µmol .018grams 0 0 0 0 0 0 0
20-2210-42 4 x 0.2 µmol .018grams 0 0 0 0 0 0 0
20-2110-41 4 x 1.0 µmol .092grams 0 0 0 0 0 0 0
20-2210-41 4 x 1.0 µmol .092grams 0 0 0 0 0 0 0

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
20-2010-01 0.1 g .1grams 0 0 0 0 0 0
20-2010-02 0.25 g .25grams 0 0 0 0 0 0
20-2010-10 1.0 g 1grams 0 0 0 0 0 0
20-2110-13 1 x 10 µmol .23grams 0 0 0 0 0 0
20-2210-14 1 x 15 µmol .345grams 0 0 0 0 0 0
20-2110-42 4 x 0.2 µmol .018grams 0 0 0 0 0 0
20-2210-42 4 x 0.2 µmol .018grams 0 0 0 0 0 0
20-2110-41 4 x 1.0 µmol .092grams 0 0 0 0 0 0
20-2210-41 4 x 1.0 µmol .092grams 0 0 0 0 0 0

References