dmf-isodG-CE Phosphoramidite

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10-1078
5'-Dimethoxytrityl-O2-diphenylcarbamoyl-N6-dimethylaminomethylidene-2'-deoxyisoGuanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C56H62N9O8P
M.W.:
1020.13
F.W.:
329.21
Catalog Number: Select Pack Size and Format to Reveal

Description

Unnatural base pairs display unique abilities in duplex DNA and in nucleic acid and protein biosyntheses. A standard Watson and Crick base pair is formed between iso-C and iso-G, but the hydrogen bonding pattern is quite different from the natural base pairs A-T and C-G. (The 5-methyl analogue was chosen as the synthetic target due to the reported instability of 2’-deoxyisocytidine caused by deamination during oligonucleotide synthesis or deprotection.)

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.|Mild Deblocking Mix Required. Use 2.5-3% DCA instead of TCA.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1078-02 0.25 g .25grams 2.45 68.33 41 25.63 18.64 13.67 3.42
10-1078-90 100 µmol .102grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1078-02 0.25 g .25grams 3.66 0.07 66.8 41.75 30.36 4.18
10-1078-90 100 µmol .102grams 1.5 0.07 23.6 14.75 10.73 1.48