dmf-dG-CE Phosphoramidite

5'-Dimethoxytrityl-N-dimethylformamidine-2'-deoxyGuanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C43H53N8O7P
M.W.:
824.92
F.W.:
329.21
CAS Number:
330628-04-1
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Glen Research CE (B-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination and tested to ensure a tight fit on synthesizers.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Controlled room temperature or lower, dry
Stability Normal


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1029-02 0.25 g .25grams 3.03 87.67 52.6 32.88 23.91 17.53 4.38
10-1029-05 0.5 g .5grams 6.06 188.67 113.2 70.75 51.45 37.73 9.43
10-1029-10 1.0 g 1grams 12.12 390.67 234.4 146.5 106.55 78.13 19.53
10-1029-20 2.0 g 2grams 24.24 794.67 476.8 298 216.73 158.93 39.73
10-1029-40 4.0 g 4grams 48.49 1603 961.8 601.13 437.18 320.6 80.15

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1029-C2 0.25 g .25grams 4.52 0.07 87.2 54.5 39.64 5.45
10-1029-C5 0.5 g .5grams 9.05 0.07 177.8 111.13 80.82 11.11
10-1029-1C 1.0 g 1grams 18.09 0.07 358.6 224.13 163 22.41
10-1029-2C 2.0 g 2grams 36.19 0.07 720.6 450.38 327.55 45.04