dmf-dG-5'-CE Phosphoramidite

3'-Dimethoxytrityl-N-dimethylformamidine-2'-deoxyGuanosine, 5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C43H53N8O7P
M.W.:
824.92
F.W.:
329.21
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Glen Research 5'-CE (ß-cyanoethyl) Phosphoramidites are designed for the production of 5'-5' or 3'-3' linkages, useful in antisense studies, or to synthesize oligonucleotide segments in the opposite sense from normal synthesis, for structural studies.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-9201-02 0.25 g .25grams 3.03 87.67 52.6 32.88 23.91 17.53 4.38
10-9201-05 0.5 g .5grams 6.06 188.67 113.2 70.75 51.45 37.73 9.43
10-9201-10 1.0 g 1grams 12.12 390.67 234.4 146.5 106.55 78.13 19.53

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-9201-02 0.25 g .25grams 4.52 0.07 84 52.5 38.18 5.25
10-9201-05 0.5 g .5grams 9.05 0.07 174.6 109.13 79.36 10.91
10-9201-10 1.0 g 1grams 18.09 0.07 355.4 222.13 161.55 22.21