dmf-5-Me-isodC-CE Phosphoramidite

5'-Dimethoxytrityl-5-methyl-N-dimethylformamidine-2'-deoxyisoCytidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C43H55N6O7P
M.W.:
798.91
F.W.:
303.21
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Unnatural base pairs display unique abilities in duplex DNA and in nucleic acid and protein biosyntheses. A standard Watson and Crick base pair is formed between iso-C and iso-G, but the hydrogen bonding pattern is quite different from the natural base pairs A-T and C-G. (The 5-methyl analogue was chosen as the synthetic target due to the reported instability of 2’-deoxyisocytidine caused by deamination during oligonucleotide synthesis or deprotection.)

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer. Mild Deblocking Mix Required. Use 2.5-3% DCA instead of TCA
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.|Note: Oligos containing 5-Me-iso-dC should be stored buffered at pH 7.5 to 8.0 to avoid decomposition.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1065-02 0.25 g .25grams 3.13 91 54.6 34.13 24.82 18.2 4.55
10-1065-90 100 µmol .08grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1065-02 0.25 g .25grams 4.67 0.07 87 54.38 39.55 5.44
10-1065-90 100 µmol .08grams 1.5 0.07 23.6 14.75 10.73 1.48