Unnatural base pairs display unique abilities in duplex DNA and in nucleic acid and protein biosyntheses. A standard Watson and Crick base pair is formed between iso-C and iso-G, but the hydrogen bonding pattern is quite different from the natural base pairs A-T and C-G. (The 5-methyl analogue was chosen as the synthetic target due to the reported instability of 2’-deoxyisocytidine caused by deamination during oligonucleotide synthesis or deprotection.)
Coupling: No changes needed from standard method recommended by synthesizer manufacturer. Mild Deblocking Mix Required. Use 2.5-3% DCA instead of TCA
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.|Note: Oligos containing 5-Me-iso-dC should be stored buffered at pH 7.5 to 8.0 to avoid decomposition.