Dithiol Serinol Phosphoramidite

3-Dimethoxytrityloxy-2-(3-((R)-α-lipoamido)propanamido)propyl-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

Product Specifications

Formula:
C44H61N4O7PS2
M.W.:
853.08
F.W.:
412.46
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

The disulfide thiol modifier may be used for introducing 3’- or 5’-thiol linkages. Dithiol Serinol, produced from lipoic acid and our patented serinol backbone, allows easy connection of multiple dithiol-labelled oligos to gold surfaces. 

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: Deprotect as required by nucleobases.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days

Intellectual Property

This product is covered by US Patent No.: 8,394,948.


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1991-02 0.25 g .25grams 2.93 84.33 50.6 31.63 23 16.87 4.22
10-1991-90 100 µmol .085grams 1 20 12 7.5 5.45 4 1
10-1991-95 50 µmol .043grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1991-02 0.25 g .25grams 4.37 0.07 81 50.63 36.82 5.06
10-1991-90 100 µmol .085grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1991-95 50 µmol .043grams 0.75 0.07 8.6 5.38 3.91 0.54