dG-CE Phosphoramidite

5'-Dimethoxytrityl-N-isobutyryl-2'-deoxyGuanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C44H54N7O8P
M.W.:
839.92
F.W.:
329.21
CAS Number:
93183-15-4
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Glen Research CE (B-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination and tested to ensure a tight fit on synthesizers.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Controlled room temperature or lower, dry
Stability Normal


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1020-02 0.25 g .25grams 2.98 86 51.6 32.25 23.45 17.2 4.3
10-1020-05 0.5 g .5grams 5.95 185 111 69.38 50.45 37 9.25
10-1020-10 1.0 g 1grams 11.91 383.67 230.2 143.88 104.64 76.73 19.18
10-1020-20 2.0 g 2grams 23.81 780.33 468.2 292.63 212.82 156.07 39.02
10-1020-40 4.0 g 4grams 47.62 1574 944.4 590.25 429.27 314.8 78.7

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1020-C2 0.25 g .25grams 4.44 0.07 85.6 53.5 38.91 5.35
10-1020-C5 0.5 g .5grams 8.88 0.07 174.4 109 79.28 10.9
10-1020-1C 1.0 g 1grams 17.77 0.07 352.2 220.13 160.09 22.01
10-1020-2C 2.0 g 2grams 35.54 0.07 707.6 442.25 321.64 44.23