dC-CE Phosphoramidite

5'-Dimethoxytrityl-N-benzoyl-2'-deoxyCytidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C46H52N5O8P
M.W.:
833.93
F.W.:
289.18
CAS Number:
102212-98-6
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Glen Research CE (B-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination and tested to ensure a tight fit on synthesizers.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Controlled room temperature or lower, dry
Stability Normal


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1010-02 0.25 g .25grams 3 86.67 52 32.5 23.64 17.33 4.33
10-1010-05 0.5 g .5grams 6 186.67 112 70 50.91 37.33 9.33
10-1010-10 1.0 g 1grams 11.99 386.33 231.8 144.88 105.36 77.27 19.32
10-1010-20 2.0 g 2grams 23.98 786 471.6 294.75 214.36 157.2 39.3
10-1010-40 4.0 g 4grams 47.97 1585.67 951.4 594.63 432.45 317.13 79.28

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1010-C2 0.25 g .25grams 4.47 0.07 86.2 53.88 39.18 5.39
10-1010-C5 0.5 g .5grams 8.95 0.07 175.8 109.88 79.91 10.99
10-1010-1C 1.0 g 1grams 17.9 0.07 354.8 221.75 161.27 22.18
10-1010-2C 2.0 g 2grams 35.8 0.07 712.8 445.5 324 44.55