dA-H-Phosphonate, TEA Salt

5'-Dimethoxytrityl-N-benzoyl-2'-deoxyAdenosine,3'-H-phosphonate, TEA salt

Product Specifications

Formula:
C44H51N6O8P
M.W.:
822.9
F.W.:
313.21
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Glen Research H-Phosphonates are analyzed by HPLC and are synthesis-tested. H-Phosphonates are especially useful for the preparation of modified internucleotide linkages which are unattainable by phosphoramidite chemistry. The most popular application is the preparation of radiolabelled phosphorothioates, since the sulfurization reaction is carried out off the synthesizer.

Details

Usage

  • Coupling: H-Phosphonate Synthesis Protocol
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Acetonitrile/Pyridine (1:1)
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability ~1week


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1200-02 0.25 g .25grams 21 686.67 412 257.5 187.27 137.33 34.33
10-1200-05 0.5 g .5grams 42 1386.67 832 520 378.18 277.33 69.33

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1200-02 0.25 g .25grams 5 0.06 93.6 58.5 42.55 5.85
10-1200-05 0.5 g .5grams 10 0.06 193.6 121 88 12.1


Product FAQs

  • What are the concentrations of the solutions used on an ABI for H-phosphonate chemistry?