Glen Research H-Phosphonates are analyzed by HPLC and are synthesis-tested. H-Phosphonates are especially useful for the preparation of modified internucleotide linkages which are unattainable by phosphoramidite chemistry. The most popular application is the preparation of radiolabelled phosphorothioates, since the sulfurization reaction is carried out off the synthesizer.
Coupling: H-Phosphonate Synthesis Protocol
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.