COT Serinol Phosphoramidite

3-Dimethoxytrityloxy-2-(3-(3-cyclooctatetraenylpropanamido)propanamido)propyl-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

Product Specifications

Formula:
C47H59N4O7P
M.W.:
822.97
F.W.:
382.35
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Bright, long-lasting and non-phototoxic organic fluorophores are essential for the continued optimization of a diverse range of imaging applications. However, all currently available technologies remain susceptible to undesirable transitions to dark states. Dark states arise from non-fluorescent triplet electronic configurations from which the rate of return to the ground state is slow. When in the triplet state, the fluorophore is susceptible to photobleaching and fluorescence applications are compromised by unpredictably reducing the signal-to-noise ratio (SNR), as well as limiting the total duration of time over which information can be gathered. The direct conjugation of small-molecule protective agents (PAs) has enabled significant improvements through intra-molecular triplet quenching. Through a partnership with Lumidyne Technologies, Glen Research has created a novel PA-linked phosphoramidite using cyclooctatetraene (COT). COT Serinol Phosphoramidite provides a means to improve the photostability of virtually any fluorophore in a modular fashion. Our spectrofluorometric studies show that the presence of COT limited the amount of photobleaching of an oligo containing the cyanine 5 dye.

Details

Usage

  • Coupling: 3 minute coupling time recommended.
  • Deprotection:  No changes needed from standard method recommended by synthesizer manufacturer. NOTE: a side-reaction can occur post-synthesis that leads to cleavage of phosphodiester linkages vicinal to the COT. To avoid this side-reaction, deprotect the oligo soon after the oligonucleotide synthesis is completed.  Otherwise, store the column at -20°C until the deprotection can be initiated.  Avoid drying down the oligo with heat.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 days

Intellectual Property

This product is covered by US Patent Nos.: 8,394,948 and 8,945,515 B2.


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1996-02 0.25 g .25grams 3.04 88 52.8 33 24 17.6 4.4
10-1996-90 100 µmol .082grams 1 20 12 7.5 5.45 4 1
10-1996-95 50 µmol .041grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1996-02 0.25 g .25grams 4.53 0.07 84.2 52.63 38.27 5.26
10-1996-90 100 µmol .082grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1996-95 50 µmol .041grams 0.75 0.07 8.6 5.38 3.91 0.54