6-oxo-6-(dibenzo[b,f]azacyclooct-4-yn-1-yl)-caproic acid sulfo-N-hydroxysuccinimide ester, sodium salt
At Glen Research, our goal was to offer a copper-free click phosphoramidite reagent with the following properties:
We are offering 5'-DBCO-TEG Phosphoramidite for preparing oligos with a 5'-DBCO modification and DBCO-dT-CE Phosphoramidite for inserting a DBCO group at any position within the oligonucleotide. DBCO-sulfo-NHS Ester is also offered for post-synthesis conjugation reactions. DBCO-modified oligos may be conjugated with azides in organic solvents, such as DMSO, or aqeous buffers. Depending on the azide used, the reaction will go to completion in 4-17 hours at room temperature. Simple desalting on a Glen Gel-Pak™ leads to a product with virtually quantitative conjugation efficiency. Note: We now recommend that synthesis of oligos containing DBCO-dT be completed using 0.5 M CSO in anhydrous acetonitrile (40-4632-xx). Acceptable results can be achieved with iodine oxidation if DBCO-dT is subjected to no more than 8-10 cycles.
|Diluent||Water and DMSO|
|Storage||Freezer storage, -10 to -30�C, dry|