tC-CE Phosphoramidite

5'-O-(4,4'-Dimethoxytrityl)-1'-(1,3-diaza-2-oxophenothiazin-1-yl)-2'-deoxy-ß-D-ribofuranosyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C45H50N5O7PS
M.W.:
835.95
F.W.:
395.33
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

The tricyclic fluorescent nucleoside analogues, 1,3-diaza-2-oxophenothiazine, tC, and 1,3-diaza-2-oxophenoxazine, tCo, are deoxycytidine analogs that have been shown to base pair faithfully with dG with virtually no disruption of the normal duplex structure.3-5 This means that the stability of the DNA duplex is not compromised as compared to the control regardless of DNA sequence. The fluorescence quantum yield of tC is essentially unchanged between single stranded and double stranded DNA - 0.21 for single stranded DNA and 0.19 for duplex DNA. Also, the fluorescence characteristics of tC are not sensitive to neighboring base combinations. tCo has been shown to be the brightest fluorescent nucleoside analogue in duplex context reported so far and even retains the majority of its fluorescence when surrounded by guanine residues. Indeed, tCo has been reported to be 25-50 times brighter than 2-aminopurine. The base analogue tCnitro is a FRET-acceptor together with tCO (or tC) as the donor molecule. This constitutes the first ever description of a nucleobase FRET-pair. This novel FRET-pair provides a unique tool for investigations of nucleic acid containing systems. tCnitro is virtually non-fluorescent under normal conditions.

Details

Usage

  • Coupling: Couping: 3 minute coupling time.
  • Deprotection: As required by nucleobases. Compatible with Ammonium Hydroxide for 17 hours at room temperature, Ammonium hydroxide/40% methylamine 1:1 (AMA) for 10 minutes at 65°C or UltraMild deprotection conditions.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 days

Intellectual Property

These products are offered under license from Ionis Pharmaceuticals, Inc. and in collaboration with ModyBase HB.


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1516-02 0.25 g .25grams 2.99 86.33 51.8 32.38 23.55 17.27 4.32
10-1516-90 100 µmol .084grams 1 20 12 7.5 5.45 4 1
10-1516-95 50 µmol .042grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1516-02 0.25 g .25grams 4.46 0.07 82.8 51.75 37.64 5.18
10-1516-90 100 µmol .084grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1516-95 50 µmol .042grams 0.75 0.07 8.6 5.38 3.91 0.54