Glen Research H-Phosphonates are analyzed by HPLC and are synthesis-tested. H-Phosphonates are especially useful for the preparation of modified internucleotide linkages which are unattainable by phosphoramidite chemistry. The most popular application is the preparation of radiolabelled phosphorothioates, since the sulfurization reaction is carried out off the synthesizer.
Coupling: H-Phosphonate Synthesis Protocol.|(The DBU is now being provided in place of the TEA salt for dC-H Phosphonate. This product shows better monomer stability and solubility.)
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.