dT-Me Phosphonamidite

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10-1130
5'-Dimethoxytrityl-2'-deoxyThymidine,3'-[(methyl)-(N,N-diisopropyl)]-phosphonamidite
Formula:
C38H48N3O7P
M.W.:
689.79
F.W.:
302.23
Catalog: Make Selections to Reveal

Description

Methyl Phosphonamidites may be used in DNA synthesizers following conventional CE Phosphoramidite protocols to produce oligonucleotides containing one or more methyl phosphonate linkages. However, deprotection and purification techniques differ and a description of the procedures is included in the Technical Bulletin. We also offer the dC monomer with acetyl base protection.1 This protecting group is removed with ammonium hydroxide during the cleavage step, eliminating modification at the dC sites during the deprotection step using ethylenediamine in ethanol.

Details

Usage

  • Coupling: 6 minutes. Note to prevent degradation of the methyl phosphonate linkage, low-water content oxidizer (40-4032) and DMAP for Cap B (40-4020) are recommended.
  • Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_Me-Phosphonamidites.pdf).
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1130-02 0.25 g .25grams 3.62 107.33 64.4 40.25 29.27 21.47 5.37
10-1130-05 0.5 g .5grams 7.25 228.33 137 85.63 62.27 45.67 11.42

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1130-02 0.25 g .25grams 5.41 0.07 101.8 63.63 46.27 6.36
10-1130-05 0.5 g .5grams 10.82 0.07 210 131.25 95.45 13.13