EDTA-C2-dT phosphoramidite contains the triethyl ester of EDTA which allows sequence-specific cleavage of single- and double-stranded DNA and RNA. The cleavage reaction is only initiated once Fe(II) and dithiothreitol are added and so is readily controlled. Coupling of EDTA-dT is normal but cleavage and deprotection should be carried out with sodium hydroxide in aqueous methanol (0.4M NaOH in methanol/water 4:1) overnight at room temperature.
Coupling: 6 minute coupling time recommended.
Deprotection: 0.4M methanolic sodium hydroxide (methanol:water 4:1) at room temperature for 17 hours.|Note: NaOH is not compatible with dmf protecting groups.