Dabcyl-dT

default
10-1058
5'-Dimethoxytrityloxy-5-[(N-4'-carboxy-4-(dimethylamino)-azobenzene)-aminohexyl-3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula:
C63H76N9O10P
M.W.:
1150.32
F.W.:
709.7
CAS Number:
316121-62-7
Catalog: Make Selections to Reveal

Description

A molecular beacon probe1 has its natural fluorescence quenched in solution unless it is hybridized to the target sequence. Consequently, the design of a molecular beacon requires a fluorophore to be in one part of the sequence and the quencher molecule to be in another, with both molecules being separated from the oligonucleotide by a hydrocarbon spacer. The Dabcyl group has been found to be a universal quencher. 3'-Dabsyl CPG and 3'-Dabcyl CPG are used to prepare probes with the quencher blocking the 3'-terminus. 5'-Dabcyl Phosphoramidite locates the quencher at the 5'-terminus and Dabcyl-dT places it within the sequence, leaving the 3'-terminus available for polymerase extension.

Details

Usage

  • Coupling: Dissolve this product in anhydrous acetonitrile:THF (9:1). |A 6 minute coupling time is recommended.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent 10% THF in Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1058-02 0.25 g .25grams 2.17 59 35.4 22.13 16.09 11.8 2.95
10-1058-90 100 µmol .115grams 1 20 12 7.5 5.45 4 1
10-1058-95 50 µmol .058grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1058-02 0.25 g .25grams 3.24 0.07 58.4 36.5 26.55 3.65
10-1058-90 100 µmol .115grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1058-95 50 µmol .058grams 0.75 0.07 8.6 5.38 3.91 0.54

References

1 S. Tyagi and F.R. Kramer, Nature Biotechnology, 1996, 4, 303-308.


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