Glen UnySupport™ 500

default
20-5040
N-Methyl-succinimido[3,4-b]-7-oxabicyclo[2.2.1]heptane-6-(4,4'-dimethoxytrityloxy)-5-succinoyl long chain alkylamino CPG

Product Specifications

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Description

Our original Universal Support (20-5000) has been discontinued since complete dephosphorylation using ammonium hydroxide, AMA or anhydrous methylamine gas takes longer than most companies wish to allocate. A recent development has been the use of a support based on a molecule which is "conformationally preorganized" to accelerate the dephosphorylation reaction.1,2 By using a rigid bicyclic molecule on the support, the rate of elimination is markedly faster than the original Universal Support. The structure of Glen UnySupport is shown below. The N-phenyl version, developed at Isis Pharmaceuticals as UnyLinker™, is available from several companies for large scale oligo synthesis. Glen UnySupport is the N-methyl version, which is preferred for high throughput oligonucleotide synthesis since methylamine rather than aniline is formed on deprotection. We are happy to offer Glen UnySupport in a variety of popular formats under license from Isis Pharmaceuticals.

Details

Usage

  • Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
  • Deprotection: Solution phase: Ammonium Hydroxide:MethylAmine (AMA) 1:1 for 1 hour at 65 C or Ammonium Hydroxide for 8 hours at 55 C. For sensitive minor bases or dyes, Glen UnySupport may be eliminated with 50 mM Potassium Carbonate in Methanol in 17 hours at room temperature or with Tert-Butylamine/water 1:3 (v/v) for 4 hours at 60 C. Gas Phase: Methylamine gas for 30 minutes at 65 C at 30 psi.
Specifications
Storage Controlled room temperature or lower, dry.

Intellectual Property

This product is covered by US Patent 7,202,264 owned by Ionis Pharmaceuticals, Inc..


References

REFERENCE(S)| 1 A.P. Guzaev, and M. Manoharan, J Am Chem Soc, 2003, 125, 2380- 2381.| 2 R.K. Kumar, A.P. Guzaev, C. Rentel, and V.T. Ravikumar, Tetrahedron, 2006, 62, 4528. (Testing text)