dA-CPG 1000

default
20-2001
5'-Dimethoxytrityl-N-benzoyl-2'-deoxyAdenosine, 3'-succinoyl-long chain alkylamino-CPG 1000
F.W.:
313.21
Catalog: Make Selections to Reveal

Description

All Glen Research CPG supports use the standard long chain alkylamino (lcaa) linker but differ in the glass pore size, 500Å, 1000Å or 2000Å. The 500Å support is appropriate for shorter sequences, while the 1000Å supports perform better in the synthesis of longer (>30-mer) DNA sequences. The 2000Å support is best for very long (>150-mer) oligonucleotides. We have instituted an additional QC test for supports to show the length of oligo that can be prepared before a drop-off in coupling due to steric effects begins to occur. The drop-off point is recorded in the Certificate of Analysis. All Glen Research supports are fully end-capped to ensure that the CPG surface is totally inert, thereby avoiding the introduction of impurity sequences containing deletions at the 3'-terminus.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: Deprotect using the protocol required by the nucleobases.
Specifications
Storage Controlled room temperature or lower, dry

Technical Documents



Product FAQs

  • How do the 1000Å and 2000Å supports compare in the synthesis of long oligos? Do you recommend any changes to the cycle for long oligos?