dC-5'-CPG

default
20-0102
3'-Dimethoxytrityl-N-benzoyl-2'-deoxyCytidine, 5'-succinoyl-long chain alkylamino-CPG
F.W.:
289.18
Catalog: Make Selections to Reveal

Description

The following supports are used to produce oligonucleotides with nuclease resistant 3’-3’ linkages at the 3’ terminus (by attaching regular 3’-CE phosphoramidites) or to produce oligonucleotide sections in the opposite sense (by attaching 5’-CE phosphoramidites). ABI-style columns are supplied unless otherwise requested (see note box).

Details

Usage

  • Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
  • Deprotection: Deprotect using the protocol required by the nucleobases. Note: Deprotection in AMA (50:50 Ammonium hydroxide/MethylAmine) may result in about 5% N4-methyl-dC side product at the modification site.
Specifications
Storage Controlled room temperature or lower, dry