2',3'-ddC-CE Phosphoramidite

default
10-7101
N4-diisobutylaminomethylidene-2',3'-dideoxyCytidine,5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula:
C27H47N6O4P
M.W.:
550.68
F.W.:
273.18
Catalog: Make Selections to Reveal

Description

To avoid polymerase extension at the 3' terminus, 2',3'-dideoxynucleoside and 3'-deoxynucleoside CPGs have proved to be effective. 2',3'- Phosphoramidites are designed to be used with the 5'-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3'-OH) groups are removed. (3'-Termination can also be effected using a 3'-3' linkage formed using 5'-supports, or 3'-spacer C3 CPG.)

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: Ammonium hydroxide at 55 °C for 17 hours.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-7101-02 0.25 g .25grams 4.54 138 82.8 51.75 37.64 27.6 6.9
10-7101-90 100 µmol .055grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-7101-02 0.25 g .25grams 6.78 0.07 129.2 80.75 58.73 8.08
10-7101-90 100 µmol .055grams 1.5 0.07 23.6 14.75 10.73 1.48