5'-OMe-dT-CE Phosphoramidite

default
10-1031
5'-O-methyl-2'-deoxyThymidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula:
C20H33N4O6P
M.W.:
456.48
F.W.:
318.22
CAS Number:
149681-62-9
Catalog: Make Selections to Reveal

Description

In situations where ligation must be blocked at the 5' terminus, 5'-OMe-dT may be used. 5'-OMe modification of a strand of siRNA using 5'-OMe-T can control guide strand selection and targeting specificity.1

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.|(Note: this product is a 5'-terminator.)
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1031-02 0.25 g .25grams 5.48 169.33 101.6 63.5 46.18 33.87 8.47
10-1031-90 100 µmol .046grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1031-02 0.25 g .25grams 8.17 0.07 157 98.13 71.36 9.81
10-1031-90 100 µmol .046grams 1.5 0.07 23.6 14.75 10.73 1.48

References

REFERENCE(S)| 1 P.Y. Chen, et al., RNA, 2008, 14, 263- 274