rSpacer TBDMS CE Phosphoramidite

default
10-3915
5'-O-Dimethoxytrityl-1'-Deoxyribose-2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C41H59N2O7PSi
M.W.:
750.99
F.W.:
196.10
CAS Number:
159299-31-7
Catalog Number: Select Pack Size and Format to Reveal

Description

rSpacer is used to introduce an abasic site to an RNA sequence.

Details

Usage

  • Coupling: 12 minute coupling recommended
  • Deprotection: RNA Deprotection-see bulletin
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 Days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3915-02 0.25 g .25grams 3.33 97.67 58.6 36.63 26.64 19.53 4.88
10-3915-90 100 µmol .075grams 1 20 12 7.5 5.45 4 1
10-3915-95 50 µmol .038grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3915-02 0.25 g .25grams 4.97 0.07 93 58.13 42.27 5.81
10-3915-90 100 µmol .075grams 1.5 0.07 23.6 14.75 10.73 1.48
10-3915-95 50 µmol .038grams 0.75 0.07 8.6 5.38 3.91 0.54